Evaluation of Salicin Isolated from Salix subserrata as a Radioprotector against Gamma Irradiation Induced Ultrastructural and Electrophoretic Changes in Spleen Tissue in Rats
Monira A. Abd El Kader*, Ibrahim Abulyazid, Mohga Shafik Abdalla, Hayat Mohamed Sharada, Wael Mahmoud Kamel
Biochemistry Department, Division of Genetic Engineering and Biotechnology, National Research Centre, 33 Bohouth st., Dokki, Giza, Egypt, affiliation ID: 60014618
Received: 16-Feb-2015 , Accepted: 30-May-2015
Keywords: Gamma irradiation, Salicin, Spleen, Protein electrophoresis, Isozymes
How To Cite
The aim of this study was to investigate the radioprotective effect of salicin against irradiation effect on spleen tissue in male rats. Lipid peroxidation product (MDA) level was measured as thiobarbituric acid reactive substance. Ultrastructural examination was carried out in spleen tissue by scanning electron microscope (SEM). The polyacrylamide gel electrophoresis for native protein, lipoprotein and zymogram were carried out in spleen homogenate. As expected, salicin resisted the irradiation effect and declined the MDA level in spleen homogenate of all treated groups. The alterations which were occurred as a result of irradiation in the spleen tissue could not be detected microscopically but they were detected electrophoertically at levels of protein and isozymes. Salicin prevented the qualitative mutagenic effect of irradiation on the electrophoretic protein pattern in the irradiated salicin simultaneous treated group (SI = 0.73). It showed the highest protective effect against qualitative mutagenic irradiation effect in catalase pattern in irradiated salicin pre-treated group (SI = 0.80). It could not prevent the abnormalities occurred qualitatively and quantitatively as a result of irradiation in peroxidase pattern in all irradiated salicin treated groups. While the esterase pattern showed the same electrophoretic pattern in the all irradiated salicin treated groups. The results suggested the radioprotective ability of salicin against gamma irradiation effect on various ultrastructural and electrophoretic patterns in spleen tissue of male rats.
Gamma rays are a packet of pure electromagnetic rays which are photons of high frequency and high energy and hence short wave length1.They can penetrate into living tissues or cells and result in transduction of radiation energy to biological materials. The absorbed energy of ionizing radiation can break chemical bonds and cause ionization of different molecules including water and different biological essential macromolecules as DNA2, membrane lipids and proteins3.
It has been reported that whole-body gamma irradiation induces oxidative stress. The most important consequences of oxidative stress are lipid peroxidation, protein oxidation and depletion of antioxidants4,5.It was found that irradiation decreases tissue concentrations of natural nonenzymatic antioxidants6,7 andcauses induction of lipid peroxidation as evidenced by increased malondialdhyde (MDA)8.
Spleen plays an important role in immune functions by proliferating lymphocytes. The integrity of the immune system depends upon the normal functioning of the lymphoid organs so that the alterations in the homeostasis of spleen tissues will affect immune responses9.It was demonstrated that spleen was the most biosensitive organs to low doses of irradiation in rats10. Irradiation caused alterations in spleen tissue and it causedinduction of DNAaffecting the radiosensitive gene in the spleen of rats11,12. Ezz,13showed that spleen taken to study the ameliorative effect of radioprotector against irradiation induced oxidative stress and immune responses in rats.
Proteins are the most complex compounds and at the same time the most characteristic of living matter. They are present in all viable cells; they are the compounds which, as nucleoproteins, are essential for cell division and, as enzymes and hormones, control many chemical reactions in the metabolism of cells. Thus, the separation and characterization of the individual proteins facilitate the study of the chemical nature and physiological function of each protein14. They are major targets for oxidative damage due to their abundance and rapid rates of reaction with a wide range of radicals and excited state species15.Changes in the protein patterns of the tissues may reflect specialization and adaptation in the organisms. It is worthy to note that each protein is considered as reflect to the activity of specific gene through the production of enzyme, which act as catalyst to produce the demanded protein; this type of produced protein is responsible for a specific biological character16.The radiation-induced alteration of the protein structure was observed by measuring the changes in the molecular properties of the proteins17. Recently, it was found that irradiation showed significant increase in protein carbonyls by 73%18.
Antioxidants enzymes as catalase (CAT) and peroxidase (GPx) are important in the elimination of free radicals19,20.They are involved to counteract the toxicity of ROS21.These enzymes are the first line of defense against oxidative injury. Superoxide dismutase is the primary step of the defense mechanism in the antioxidant system against oxidative stress by catalyzing the dismutation of 2 superoxide radicals (O2-) into molecular oxygen (O2) and hydrogen peroxide (H2O2)22. H2O2 can synthesize a highly reactive OH, is neutralized by the combined action of CAT and GPx in all vertebrates23,24.These enzymes act in coordination and the cells may be pushed to oxidative stress state if any change occurs in the levels of enzymes21.
Irradiation can exert a significant inflammatory response in cells. So, it is essential to develop methods to target the radiosensitive organs and / or to protect the normal tissues. Antioxidants eliminate the free radicals and neutralize reactive oxygen species (ROS) before they can do their damage. However, much remains unknown about mechanisms of radio-protection. Development of protective agents presented new solutions for recovery of undesired tissue damage induced by irradiation25,26.
The discovery of radioprotectors for the first time seemed to be very promising and has attracted the interest of a number of radiobiologists. Although synthetic radioprotectors such as the aminothiols have yielded the highest protective factors; typically they are more toxic than naturally occurring protectors27.
Salicin (C13H18O7) is a natural product extracted from several species of Salix (willow) and Populus (poplar), and was also found in Gaultheria procumbens (wintergreen) and in Betula lenta (sweet birch), the volatile oils of which consist almost entirely of methyl salicylate28.Salicin is considered as natural aspirin. It is very possible to be digested without side effects in the stomach and kidneys, while acetylsalicylic acid is known to upset the stomach and in some cases damage kidneys. Scientists believe that this is because salicin is converted to acetylsalicylic acid after the stomach has absorbed it29.It is a pro-drug that is gradually transported to the lower part of the intestine, hydrolysed to saligenin by intestinal bacteria, and converted to salicylic acid after absorption. It thus produces an antipyretic action without causing gastric injury30.
It belonged to the phenolic compounds which are believed to work synergistically to promote healthy conditions through a variety of different mechanisms, such as enhancing antioxidant activity, impacting cellular processes associated with apoptosis, platelet aggregation, blood vessel dilation, and enzyme activities associated with carcinogen activation and detoxification31,32.
The present main objective is to optimize salicin as a radioprotector against effect of gamma irradiation on the spleen tissue in the hope that this compound may be further explored as novel antioxidative radioprotector.
2 Materials and Methods
2.1 Salicin isolation
Salicin was extracted and isolated from fresh young leaves of willow trees (Salix subserrata, Salix safsaf) according to method suggested by Mabry et al. and purified according to method described by Kur`yanov et al.34 then identified qualitatively by advanced chromatographic techniques.
2.2 Acute toxicity test
The safety of salicin orally was evaluated by determination it’s LD50. Forty eight adult female albino mice weighing 20-25 g was used to study acute toxicity. It was divided into 6 groups each of 8 mice. The groups were treated orally with rising doses of 500, 1000, 2000, 3000, 4000 and 5000 mg/kg body weight of aqueous solution of salicin solution. Mortality was recorded 24 hrs post treatment. The LD50 was calculated according to the equation suggested by Paget and Barnes35.
Seven groups of male rats weighing between 150-200 gm per one obtained from the animal house laboratory of national research centre. Ten rats in each group. All the animals were kept under normal environmental and nutritional conditions. The animal groups were divided into Rats were non-irradiated and non-treated with salicin representing Control group; Rats were non-irradiated but treated with the safe dose of salicin (was about 150 mg / Kg) taking in the consideration weight of each rat representing Salicin treated group; Rats were irradiated at the dose 7 Gy and non-treated with salicin representing Irradiated group; Rats were treated with salicin for 15 days followed by irradiation at the 15th day representing Irradiated salicin pre-treated group; Rats were treated with salicin for 15 days followed by irradiation at the 15th day then the treatment was continued daily for another 15 days representing Irradiated salicin prepost-treated group; Rats were irradiated and treated with salicin at the same time of irradiation and continue daily for 15 days representing Irradiated salicin simultaneous treated group; and Rats were irradiated at the same gamma dose then left without treatment for 15 days. At the 15th day, the rats were treated with salicin for another 15 days representing Irradiated salicin post-treated group.
Whole body of the animals was exposed to an acute single dose of 7 Gy delivered at a dose rate of 1.167 Rad/Sec. using cobalt-60 (Co60) from the biological irradiator gamma cell source belonging to Middle Eastern Regional Radioisotopes Center for Arab Countries, Dokki, Cairo, Egypt.
2.5 Lipid peroxidation measurement
Lipid peroxidation level was measured as thiobarbituric acid reactive substance in spleen homogenate according to method of Ohkawa et al.36.
2.6 Scanning Microscopic examination (SEM).
This examination was carried out in piece of spleen tissue using SEM. The tissue was preserved in gluteraldhyde purchased from Gpr Chemicals Co. It was prepared according to the method suggested by Tánaka37 who reported that the specimen showed be passed through series of the dehydration steps by placing it in ethyl alcohol then incubated for 15 min. then coated with the golden atoms to be ready for the electron microscopic examination.
2.7 Electrophoretic protein and lipoprotein patterns
Total protein was determined in spleen homogenate according to Bradford38.The sample was mixed with the sample buffer. The protein concentration in each well must be about 70 μg protein. Proteins were separated through polyacrylamide gel electrophoresis (PAGE). Electrode and gel buffer and polyacrylamide stock were prepared according Laemmli39.After electrophoretic separation, the gel was gently removed from the apparatus and put into a staining solution of coomasie brilliant blue for native protein pattern40and staining solution of sudan black B (SBB) for lipoprotein pattern41.
Native protein gel was stained for peroxidase pattern using certain stain prepared according to the method suggested by Rescigno et al.42, for catalase pattern according to method described by Siciliano and Shaw43 and for esterase pattern according to method of Baker and Manwell44.
2.9 Data analysis
The polyacrylamide gel plate was photographed, scanned and then analyzed using Phoretix 1D pro software (Version 12.3). The similarity index (S.I.) compares patterns within, as well as, between irradiated and non-irradtated samples. The similarity values were converted into genetic distance (GD) according the method suggested by Nei and Li45.
3 Statistical Analysis
All the grouped data were statistically evaluated with SPSS/16.00 software. The results were expressed as mean ± SE of studied groups using the analysis of variance test (one-way ANOVA) followed by student’s t-test. P values of less than 0.05 were considered to indicate statistical significance. The means of irradiated groups and the salicin treated groups were individually compared with those of control group. The irradiated group was compared with irradiated salicin treated groups.
4.1 Lipid peroxidation
As compared to control, irradiation caused significant (P < 0.05) elevation in the MDA level in spleen tissue. Salicin administration showed the ameliorative effect against irradiation by reducing MDA level in all irradiated salicin treated rats. From the data compiled in Table 1, it was found that salicin showed the most suitable antagonistic effect against irradiation on spleen of irradiated salicin pre-treated group as compared to irradiated group.
4.2 Spleen ultrastructure
The ultrastructural observations in spleen tissue of control rats revealed normal tissue surface (Fig. 1a). Salicin administration showed normal appearance, no ultrastructural changes and no deviation from control (Fig. 1b). Irradiation caused no obvious abnormalities on the spleen surface indicating to inability of this radiation dose to cause any differences observed microscopically on surface of the spleen tissue (Fig. 1c). In the irradiated salicin pre-treated group, there was superfacial lesions with cellular loses (Fig. 1d).
Spleen tissue showed smooth appearance in irradiated salicin simultaneous treated group with presence of blood aggregates on the tissue surface (black arrow) (Fig. 1e). In the irradiated salicin prepost-treated group, it was found that there was surface erosion (red arrow) with presence of blood aggregates (black arrow) (Fig. 1f).The irradiated salicin post-treated group displayed deep cracking with widening the gap between the cells (Fig. 1g).
4.3 Electrophoretic protein pattern
As shown in Table 2 and illustrated in Fig. 2, there were no common bands in all groups but there was one characteristic band appeared in irradiated salicin post-treated group with Rf 0.32 (Mwt 55.32 KDa and B% 7.13). Irradiation caused severe qualitative mutation represented by disappearance of 2 bands with deviation of the 3rd, 4th, 5th and 6th bands to be appeared with Rfs 0.21, 0.48, 0.58 and 0.64 (Mwts 121.29, 25.82, 18.82 and 16.84 KDa) respectively. Salicin showed the highest antagonistic effect against the qualitative mutagenic effect of irradiation in the irradiated salicin simultaneous treated group. It could not prevent the quantitative mutation which was represented by increasing B% of the 5th normal band (Rf 0.63, Mwt 17.16 KDa and B % 55.42).
From the SI values, it was found that the lowest SI value (SI = 0.15) was recorded with irradiated salicin post-treated group and the highest SI value (SI = 0.67) was recorded with irradiated salicin pre-treated and simultaneous treated groups. As compared to SI value of the irradiated group (SI = 0.20), salicin prevented the irradiation effect in all groups except irradiated salicin post-treated group.
4.4 Electrophoretic lipoprotein pattern
Lipoprotein pattern showed thatthere were 2 common bands appeared in all groups with Rfs 0.04 and 0.97 (B % 23.77 and 35.31) (Table 3 and illustrated in Fig. 3).
Irradiation caused alterations represented by disappearance of 2 normal bands with deviation of normal band to be appeared with Rf 0.13 (B % 28.51). There was no quantitative mutation.Salicin could not prevent the irradiation effect represented by deviation of 2 normal bands to be appeared with Rf 0.13 and 0.51 (B % 20.30 and 12.42) in the irradiated salicin pre-treated group, by deviation of one normal band to be appeared with Rf 0.11 (B % 14.35) with appearance of one abnormal band with Rf 0.33 (B % 11.64) in the irradiated salicin simultaneous treated group and represented qualitatively by disappearance of one normal bands with deviation of the 2nd normal band to be appeared with Rf 0.11 (B % 20.81) and quantitatively by increasing B % of the normal band appeared with Rf 0.98 (B % 59.39) in the irradiated salicin post-treated group. While in the irradiated salicin prepost-treated group, salicin could not prevent the alterations represented qualitatively by disappearance of 2 normal bands and quantitatively by increasing B % of the normal bands appeared with Rfs 0.04 and 0.99 (B % 37.94 and 62.06).
From the SI values, salicin decreased the irradiation effect on the band number and arrangement in the irradiated salicin pre-treated (SI = 0.60) and simultaneous treated groups (SI = 0.73).
4.5 Electrophoretic esterase pattern
As shown in Table 4 and illustrated in Fig. 4, there were 3 common bands appeared in all the groups with Rfs 0.18, 0.36 and 0.80 (B % 21.52, 49.21 and 21.37). Irradiation caused no quantitative mutation but it caused qualitative alterations represented by disappearance of the 3rd normal type of the enzyme pattern without appearance of abnormal bands. As compared to control, irradiation showed the same electrophoretic esterase pattern in the irradiated and all irradiated salicin treated groups. The SI values were equal in all irradiated salicin treated groups (SI = 0.86). There was complete similarity between these groups. The highest SI value (SI = 1) was recorded with the salicin treated group.
4.6 Electrophoretic catalase pattern
The electrophoretic catalase pattern showed that there were no common bands appeared in all groups (Table 5 and illustrated in Fig. 5).
Irradiation caused qualitative alteration represented by disappearance of one normal type with appearance of one abnormal band with Rf 0.28 (B % 69.42). Salicin administration showed the highest protective effect against qualitative mutagenic effect of irradiation in the irradiated salicin pre-treated group. It could not prevent the irradiation effect which was represented qualitatively by appearance 2 abnormal bands with Rfs 0.28 and 0.59 (B % 19.45 and 29.30) in the irradiated salicin simultaneous treated group, with Rfs 0.16 and 0.27 (B % 34.62 and 25.57) in the irradiated salicin prepost-treated group and Rfs 0.20 and 0.57 (B % 32.77 and 26.19) in the irradiated salicin post-treated group. It could not prevent the quantitative mutation which was represented by decreasing the B % of the 1st type of the catalase enzyme in all irradiated salicin treated groups.
The SI values showed that the lowest SI value (SI = 0.33) was observed with irradiated salicin simultaneous group and the highest value (SI = 0.8) observed with irradiated salicin pre-treated group. In the irradiated salicin prepost-treated group, it was observed that all the bands were not matched with all bands of the other groups. Salicin treatment minimized the irradiation effect the irradiated salicin pre-treated (SI = 0.80) and post-treated group.
4.7 Electrophoretic peroxidase pattern
All the data were recorded in Table 6 and illustrated in Fig. 6. There were no common bands in all groups. Irradiation caused qualitative alterations represented by disappearance of the 1st and 6th types with appearance of one abnormal band with Rf 0.27 (B % 30.98) and deviation of the 2nd, 3rd and 5th types to be appeared with Rfs 0.33, 0.40 and 0.62 (B % values 8.14, 9.02 and 18.89). Salicin administration could not prevent the abnormalities occurred qualitatively and represented by disappearance of normal bands, appearance of abnormal bands and deviation of some normal bands to be appeared with different data. It could not prevent the alterations which were represented quantitatively in all irradiated salicin treated groups by increasing B % of some normal bands.
The SI values showed that the lowest SI value (SI = 0.15) was observed in irradiated group and the highest value (SI = 0.43) noticed in the salicin treated group. In the irradiated salicin pre-treated group, it was observed that all the bands were not matched with all bands of the other groups.
Spleen of the male rats was selected to be under study due to sensitivity of the male rats to irradiation damages more than female rats. This was in agreement with Ezz, (2011)13who showed that spleen taken to study the ameliorative effect of radioprotector against irradiation induced OS and immune responses in male rats.
During results of the present study, the MDA level elevated significantly as a result of radiation exposure in spleen tissue. This was in accordance with the results obtained by Dixit et al. (2012)46 who reported that irradiation at the doses 2, 6 and 10 Gy enhanced the MDA level. This may refer to increasing in intracellular ROS concentration which leads subsequently to oxidative stress47 and decrease in activity of antioxidant enzymes with possible damage of cellular membranes48. Kergonou et al. (1981)49 showed that the MDA level increased after radiation exposure due to radiosensitivity of spleen and also because the MDA is released from tissues in plasma and trapped from plasma in kidney and spleen.
Although it is well known that irradiation induced cellular injury due to the harmful effects of the free radicals which play a key role in irradiation induced apoptosis50,it caused no obvious alterations detected microscopically during the current study.
During the current study experiment, it was showed severe alterations detected electrophoretically at level of protein and isozymes although irradiation showed no morphological alterations on the spleen surface. This might refer to the irradiation effect which caused DNA strand breaks due to increased production of ROS which attack DNA in splenocytes and / orthe effect on radiosensitive gene in spleen tissue12.
Ramanathan and Misra (1979)52 reported thatirradiation induced changes in lipid metabolism in spleen of rats. The fatty acid composition of spleen was profoundly altered 24 hrs after irradiation. Free radicals implicated in OS reactions, which can damage cells and tissues and cause disorders in the immune system53.
As reported by Hamzaa et al. (2012)54, it was found that the phenolic compounds have ameliorating effects against oxidative damage induced by gamma-irradiation through inhibition of lipid peroxidation, improvement of lipid profile and enhancement of the antioxidant activity.
Salicin hydrolyzes in the gastrointestinal tract to give D-glucose and salicyl alcohol. Upon absorption, salicyl alcohol is oxidized into salicylic acid55.Thus in the current study, the effect of salicin was attributed to its hydrolysable form salicylic acid.
The effect of salicylic acid was compatible with an antioxidant profile: it inhibited lipid peroxidation and increasedglutathione synthesis, but did not modify the activitiesof glutathione-related enzymes56. The effect of the salicylic acid on lipid peroxidation may be explainable by the ability of salicylic acid to absorb hydroxyl ions57 and thus impede a main step in the process of membrane lipid peroxidation. Salicylic acid might spare glutathione stores by avoiding factors that stimulate glutathione depletion. Two observations support this notion: the percentage of oxidized glutathione was reduced, and the activities of enzymes associated with maintaining glutathione levels were not modified substantially56.Salicylic acid showed a direct effect on the glutathione system. This effect may be related with the ability of both to react with hydroxyl radicals57,58.
On the other hand, Rebouch and Seim (1998)59 and Ibrahim et al. (2007)60recorded thatsalicin might induce elevation in activities of the AOs as glutathione peroxidase in these tissues. It might act by improving the turnover of fatty acids peroxidated by the free oxygen radicals during normal metabolism. It might be added to category of the natural products as olive oil, Nigella sativa oil and pomegranate extract which play vital role in male fertility61.
The present results showed that irradiation caused alterations in all electrophoretic patterns in spleen tissue. This was in agreement with results reported by many previous studies which suggested that irradiation produces ROS that damage proteins, lipids and nucleic acid62.
The current experiment showed that irradiation decreased the ordered structure of proteins. This was in agreement Moon and Song, (2001)17 who suggested that radiation caused initial fragmentation of polypeptide chains and, as result, subsequent aggregation and degeneration of proteins by scavenging ROS produced by irradiation. The difference in the protein fractions separated electrophoretically after radiation exposure was in agreement with Pleshakova et al., (1998)63who reported that irradiation caused a rise of protein carbonyl only in the cytoplasm and mitochondria and this was followed by activation of histone – specific proteases in nuclei of the irradiated rats. The proteins are responsible for a specific biological process, so due to the difference in protein bands between all the treated samples, the biological processes may also be differed. The separation and characterization of the individual proteins facilitate study of the chemical nature and physiological function of each protein64.
During the current study, irradiation caused alterations in the native proteins detected electrophoretically. This was in accordance with Davies and Delsignore (1987)65who documented that irradiation caused irreversible changes at the molecular level by breakage of the covalent bonds of the polypeptide chains due to generation of the hydroxy and superoxide anion radicals which modify the primary structure of the proteins resulting in distortions of the secondary and tertiary structures. The exposure of proteins to oxygen radicals resulted in both non-random and random fragmentations66. It was reported that irradiation caused aggregation and cross-linking of proteins. Covalent cross linkages are formed between free amino acids and proteins, and between peptides and proteins in solution after irradiation66,67.
Data in the present study indicated that specific protein bands in spleen tissue of irradiated rats differed (through disappearence in some protein bands or appearance of new ones). Disappearance of some protein bands in treated rats may be attributed to the effects of irradiation which inhibits the synthesis and expression process of these deleted proteins (qualitative effect). In addition, even the band remained after irradiation, it usually differs in the amount of protein, and this may be explained by that irradiation could not inhibit the synthesis of this protein type, but it may be affected only on the quantitative level.
Giometti et al. (1987)68postulated that different mutations were detected by the appearance of new proteins or by the quantitative decrease in abundance of normally occurring proteins and the electrophoresis can be used to detect the mutations reflected as quantitative changes in the protein expression.
The difference in protein pattern may act as a tool to identify the similarity index and genetic distance between the control and the other treated samples. The chemical changes of the proteins that are caused by irradiation are fragmentation, cross-linking, aggregation, and oxidation by oxygen radicals that are generated in the radiolysis of water69.
Lipoproteins are lipid–protein complexes that contain large insoluble glycerides and cholesterol with a superficial coating of phospholipids and proteins synthesized in the liver70. All lipoproteins carry all types of lipid, but in different proportions, so that the density is directly proportional to the protein content and inversely proportional to the lipid content71.
In the present study, irradiation caused alterations in the electrophoretic lipoprotein pattern. This was in agreement with Tsumura et al. (2001)72who reported thatthe lipoproteins were more susceptible to oxidative modifications resulting in small lipoproteins.
Bonnefont-Rousselot (2004)73mentioned that the ROS can initiate one-electron oxidation or one-electron reduction reactions on numerous biological systems. The oxidative hypothesis classically admits the involvement of the lipoproteins oxidation radiolytically.
There was natural binding between protein and lipoproteins in the rat tissues. These two tissues known to be involved in the processing of the lipoproteins. The lipoproteins-binding protein has previously been identified in adrenal cortical plasma membranes and concentration of the binding protein was strongest in kidneys74. So the alterations in the protein pattern were associated with altering the lipoprotein pattern in these tissues. The alterations in the lipoprotein pattern may refer to the disturbances in the cholesteryl esterase required or cholesterol hydrolysis75.
Esterases are very large class of enzymes. They can break an ester bond in the presence of water molecule76.The esterase activity stimulated breakdown of acetylcholine liberated during nervous stimulation. They are very polymorphic, tissue-specific and variable in populations of rats. Esterase zymograms showed that intensity and number of the nonspecific esterase bands are very variable77.Esterases are found associated with membrane structures. There was correlation between different esterases and the total esterase activity in the different tissues78.
According to results of the present study, irradiation caused electrophoretic qualitative and quantitative alterations in the electrophoretic esterase pattern in the spleen tissue. This may refer to effect of irradiation on the protein pattern79or the disturbances occurred in the cholesterol metabolism as a result of radiation exposure. The total esterase activities were correlated to the cholesterol responses in rats80.
As regards changes in electrophoretic mobility demonstrated in the present study, it seemed that free radicals affect the integrity of the polypeptide chain in the protein molecule causing fragmentation of the polypeptide chain due to sulfhydral-mediated cross linking of the labile amino acids as claimed by Bedwell et al. (1989)79. The changes in the fractional activity of different isoenzymes seemed to be correlated with changes in the rate of protein expression secondary to DNA damage initiated by free radicals81.
During the current experiment, irradiation caused alterations in the electrophoretic catalase and peroxidase patterns. This was in agreement with Li et al. (2007)82whoshowed that irradiation decreased the peroxidase activity which may be due to that irradiation-induced ROS markedly alters the physical, chemical and immunologic properties of endogenetic antioxidant enzymes (CAT and GPx), which further increase oxidative damage in cells.
The study showed that the decrease in CAT and GPx activity could be attributed to the uncontrolled production of ROS and accumulation of H2O2 whereby oxidative damage to enzymes can cause a modification of their activity83,84.
Bhatia and Manda (2004)85reported that the electrophoretic disturbances occurred as a result of irradiation in the peroxidase pattern. This might be due to irradiation-induced depletion in the level of reduced GSH, as well as GSH peroxidase. This leads to elevation of the hydrogen peroxide and hence generation of the free radicals86.GPx utilizes GSH as a substrate to catalyse the reduction of organic hydroperoxides and H2O287.
Salicin and salicylic acid belonged to the phenolic compounds which showed antioxidant activity due to their ability to scavenge free radicals88.The maintenance of normal protein levels after the treatment with salicin may be due to trapping of these free radicals by this compound, thus preventing DNA damage. Salicin was able to overcome the disturbances in the protein pattern in the spleen tissue. It showed protective effect against the irradiation due to its antioxidative effect against attack of the free radicals. It prevented the alterations in the proteins and hence the lipoproteins and isozymes in the spleen tissue.
The current results are in line with that obtained by Cetin et al. (2008)89 who suggested that salicin treatment considerably increased the formation of antioxidant products in different tissues. Salicin treatment minimized the irradiation effect and this may refer to its effect on stimulation of activities of the different enzymes. The mRNA expression levels of the enzymes increased after administration of salicin whicjh may play role in regulation of these enzymes on a transcriptional level90.
The study concluded that salicin minimized the irradiation effect and showed radioprotective effect against irradiation induced ultrastructural and different electrophoretic changes in spleen tissue of male rats.
7 Competing interest
The present study aimed to optimize salicin as a radioprotector against effect of gamma irradiation on the spleen tissue in the hope that this compound may be further explored as novel antioxidative radioprotector.
8 Author’s contributions
MALAK and MSA carried out literature review and draft the manuscript. HMS participated in collection of data and arranged in tabular form. IA and WMK carried out the experimental work. All authors read and approved the final manuscript.
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