Changes in Seedling Growth and Biochemical Contents in Abrus precatorius L. Under Nickel Treatment


Milvee K. Vyas

Department of Biosciences, Veer Narmad South Gujarat University, Udhna Magdalla Road, Surat, 395007, Gujarat, India


An attempt was made to study the effect of nickel on the growth of Abrus precatorius, a medicinal plant growing in South Gujarat used in Ayurved. The seeds of Abrus precatorius were germinated in petri dishes by 25, 50, 100, 150, 200 ppm nickel sulphate solution for experimental work.  The germination percentage, seedling growth, dry matter yield and changes in biochemical contents of total sugar, protein and pigments were investigated ten days after showing. The study indicates that the lower level of nickel has no adverse effect on germination, seedling growth and biochemical content whereas the higher concentration decreased the same accept the protein. Nickel increased the protein content at lower concentration and has been reducing effect at higher level. 


Determination of Prevalence of Urinary Tract Infection Among the Pregnant Women with Lower Abdominal Pain


Chandra Bala Sekharan1*, Devarajan Dinesh Kumar2, Koneru Ratna Kumari1, Cecilia Alphonce Joachim1

1Department of Biochemistry, International Medical and Technological University, Dar Es Salaam, Tanzania - 77594

2Department of Anatomy, International Medical and Technological University, Dar Es Salaam, Tanzania - 77594


The present study was aimed to determine the prevalence of urinary tract infection among the pregnant women with lower abdominal pain and its aetiological micro-organism. Cross sectional study was done at Mount meru hospital laboratory, Arusha, Tanzania.  225 pregnant women attending an antenatal clinic in Levolosi hospital (Arusha, Tazania) were enrolled. To diagnose urinary tract infection in the enrolled participants, mid stream urine was collected and culture on Macconkey agar media and blood agar media. Urine analysis was done using dipstick test, urine microscopy and biochemical tests. The prevalence of urinary tract infection was found to be 31.6%.  High incidence of infection was found in 33-40 years age group (41.6%). The incidence of infection was high in the third trimester of pregnancy compared to first and second trimester. The prevalence of infection is more in participants who had past history of infection. The bacterial pathogens isolated include E.coli (40.8%), Staphylococcus species (30.0%), Klebsiella species (14.1%), Proteus species (11.3%), Citrobacter species (1.4%) and Enterobacter species (1.4%). Diagnosis of urinary tract infection in pregnant women during all antenatal visits should be considered a vital care in the community. This helps to keep away from complications in pregnancy at an early stage. 


Antimicrobial Susceptibility Pattern of Fosfomycin in Various Clinical Isolates


Humaira Zafar1*,  Nouman Noor2, Kiran Tauseef Bukhari3, Sadaf Humayun2, Noor Khan Lakhnana4

1Department of Microbiology, Al Nafees Medical College & Hospital, Islamabad

2Department of Dentistry, Rawal Dental College, Rawal Institute of Health Sciences, Islamabad

3Department of Haematology, Al Nafees Medical College & Hospital, Islamabad

4Department of Pathology, Al Nafees Medical College, Islamabad.


To identify the susceptibility pattern of Fosfomycin in various clinical isolates by estimating the frequency in terms of percentages. A convenient sampling technique was adopted for study proceedings. Total 748(n) specimens for culture and sensitivity were received in the microbiology section of pathology department. Out of these 748(n) specimens, positive cultures were seen in 144(n). For culture and sensitivity proceedings, the recommended CLSI – 2014 (clinical and laboratory standard institute) guidelines were followed. The bacterial isolation was done by biochemical tests. The zone diameter of >16 mm for 50µgm fosfomycin disc was considered as sensitive zone. While <15-12mm was considered as intermittent one and


Radiation Response (Low Dose Gamma Radiation) of Members of the Enterobacteriaceae in Dry Fruiting Bodies of Pleurotus ostreatus and Their Identification Using API 20E System


Nii Korley Kortei1*,George Tawia Odamtten2,MaryObodai3, Michael Wiafe- Kwagyan2

1Department of Nutrition and Dietetics, School of Allied Health Sciences, University of Health and Allied Sciences, PMB 31, Ho, Ghana

2Department of Plant and Environmental Biology, College of Basic and Applied Sciences, University of Ghana, P. O. Box LG 55, Legon, Ghana

3Food Microbiology Division, Food Research Institute - Council for Scientific and Industrial Research, P. O. Box M20, Accra, Ghana


The effectiveness of using low dose gamma radiation (1, 2, 3, 4 and 5 kiloGray) was tested on dry fruit bodies of Pleurotus ostreatus stored in polythene and polypropylene and stored at 28-30 oC up to 12 months for identification of members of Enterobacteriaceae with API 20E test kit (Biomerieux ®, France). Encountered microorganisms of the Control samples (0 kGy) of both packages recorded included Klebsiella pneumoniae, Citrobacter freundii, Pseudomonas aeroginosa Proteus mirabilis, Serratia marscesens and Enterobacter spp.  There was an observed decrease in bacteria species with increasing dose. However, Klebsiella pneumonia persisted after the application 5 kGy dose in both packaging materials. Health and immuno compromised persons stand a greater health risk after interaction with some of these bacteria spp. Case in point, infection of patients with cystic fibrosis by Pseudomonas aeruginosa is known to be lethal over a long period of time. Information on Enterobacteria in the food industry is essential since they are potential food poisoning agents and thus their control is imperative. 


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